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ÿþWe recently reported a rihanna puma slides new UV-curable polyurethane-methacrylate (PUMA) resin that has excellent qualities as a disposable microfluidic substrate for clinical diagnostic applications. This article discusses strategies to improve the production yield of PUMA chips that contain dense and high-aspect-ratio features, which presents unique challenges in demolding and bonding steps. These fabrication improvements were deployed to produce a microfiltration device that contained closely spaced and high-aspect-ratio columns.

Certified by the supplier as United States Pharmacopeia (USP) Class VI-compliant, this PUMA resin has been tested thoroughly for its chemical inertness, working temperature, biocompatibility, and sterilizability  all qualities necessary for manufacturing medical diagnostic devices.In this article, we focus on the back-end steps  demolding, bonding, and puma fenty slides interconnecting to external fluidic delivery  of producing a PUMA chip. During demolding, high-aspect-ratio microstructures are prone to shear-induced damage.

Providing nominally 80 mW/cm 2 at 365 nm) for 80 sec (expose through resin side), followed by an puma shoes for women additional 40 sec (expose through mold). Once released from the mold, PUMA substrate was conformally bonded to another PUMA-coated (cured) glass coverslip with gentle mechanical pressure. This conformal bond was converted to permanent bond by placing the PUMA chip under the UV flood source for an additional 10 min. Material layout during molding of PUMA chip. 1 : PDMS mold; 2 .

Causing delamination from the adhesive. The best tubing among those evaluated was the 1/16-in OD PTFE rihanna puma shoes tubing. Although it is nearly impossible to chemically bond to PTFE tubings, that can be circumvented by covering the external surface with a polyolefin heat-shrink. Then the PTFE tubing may be inserted either directly into a 1/16-in diameter hole and secured with additional resin, or into a 1/8-in hole with a supplemental PU tubing (1/8-in OD) as a shear anchor, and lastly secured with additional resin.

Laser power, and spot focus need to be fine-tuned to avoid excessive melting. The sputtering problem should be readily addressable with a protective mask ( e.g. cellophane or Scotch tape) on top of critical features.Cured PUMA resin has a Shore hardness of D 60, which is significantly harder than the elastomeric PDMS (Shore A 50 for Dow Corning's Sylgard 184). For free standing, mechanically puma blaze of glory fragile features (in particular unsupported tall vertical columns or whiskers).